A research team is attempting to silence the expression of a specific disease-associated gene without altering its underlying genomic DNA sequence or inducing double-strand breaks (DSBs). Which of the following approaches is most appropriate for achieving stable, reversible transcriptional repression?
- Wild-type SpCas9 co-expressed with a single-guide RNA (sgRNA)
- Catalytically dead Cas9 (dCas9) fused to a DNMT3A DNA methyltransferase domain
- High-fidelity Cas12a targeting exon coding sequences
- Zinc Finger Nucleases (ZFNs) engineered for target cleavage
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